The aim of this paper is to analyze sustainable tourism in the City of Zagreb and to strengthen the development possibilities of specific forms of tourism as a measure of sustainable development of tourism in the City of Zagreb. Europe as the leading tourist region of the world has good foundations and tradition of tourism and, accordingly, facilitates further development of various forms of tourism. The paper analyzes the development of the tourism policy of the EU and the City of Zagreb, and measures and activities aimed at the sustainable development of tourism in the City of Zagreb in accordance with national and European policies. Special attention is paid to the development of specific types of tourism such as ecotourism, sports and recreational tourism with special emphasis on cyclotourism, youth tourism, cultural tourism, enogastronomic rural tourism in the City of Zagreb. Secondary sources of data were used in this paper, where relevant scientific and professional literature was studied and various internet sources were also studied. In order to determine how much the public perceives Zagreb as a sustainable destination for sustainable tourism for research purposes, an empirical survey was conducted through a survey questionnaire. The research has shown that the public is very positive about the possibility of developing and strengthening sustainable tourism in the City of Zagreb.