Thesis gives the description of motivation as one of the key factors in hotel business success. Motivation is an encouragement in everyone to accomplish goals, in this case organization goals and to make business better. In the first part of the thesis is given the description of organization of human potential, work structure and development of staff career. Second part gives the definition of motivation and lists content and process motivational theory. There are many methods to make work more efficient, which are described through material and nonmaterial components of motivation. Results of the survey conducted on the visitors of hotel Laguna Materada in Poreč are brought in the last part of the thesis. H1 and H2 are proven through research of the visitors, more precisely, business success is expressed through guest’s expectations and satisfaction, providing a good service is an important element for success. Quality service is influenced by employee’s motivation. Thesis contains graphs, tables and surveys that are given guests to fulfill. The purpose of this thesis is to show the impact of motivation as an important factor in a successful business examples. It is also important to show how organization and management interactions influence employee’s motivation. The aim of this thesis is derived from hypothesis and research subject. Therefore, we must understand the importance of employees in organization hierarchy and the influence of their motivation to the products of their work, in this case a quality hotel service which makes a guest pleased. Hypothesis 1: Business success is expressed through guest’s expectations and satisfaction. (Proven) Hypothesis 2: Business success is expressed through quality service, which is influenced by employee’s motivation (Proven)